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The human cytokeratin 20 (CK20) gene, a member of the cytokeratin gene family, is expressed in a variety of epithelial tissues and appears to be a sensitive indicator of human cancer. We report that the expression of the human CK20 gene is controlled by the retinoic acid response element (RARE) in the proximal promoter region. Site-directed mutagenesis of RARE and gel shift assays were used to show that the RARE present at -2059 to -2035 nt is crucial for the retinoic acid responsiveness of the CK20 promoter in both human cervical carcinoma Hela cells and mouse melanoma B16 cells. To further understand the regulation of the CK20 promoter by the RARE, we analyzed the nuclear extracts of Hela cells with various treatments. Treatment of the cells with retinoic acid and 12-O-tetradecanoylphorbol-13-acetate (TPA) together with retinoblastoma binding proteins results in a dramatic reduction of the retinoic acid responsiveness of the CK20 promoter. 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It allows you to start and stop timesheets, set up groups and users for your employees, create reports and export data to Excel. It's so easy to use you'll be amazed. Key Features: * Add, delete, change, and duplicate users, groups and computers for your project * Add, delete, change and duplicate groups * Share groups, users, computers, and projects with other computers on your network * Setup group restrictions * Create time sheets and schedules for users and groups * Set up the way to start and stop timesheets for each project * Start and stop timesheets on a computer or group basis * Set group start and stop times and group exceptions * Show detailed start and stop times for each group * Generate detailed reports on user or group timesheets, projects or calendars * Export data to text, csv, xlsx, xlsm, pdf, txt, rtf, and html * Import data from csv, xlsx, xlsm, pdf, txt, rtf, and html * Import from Microsoft Access * Import from other programs * Set user and group passwords for protection * Export User and Group passwords to a text file * Export User and Group master data to text files * Export User, Group, Computer and Computer Group master data to a text file * Export User, Group, Computer and Computer Group to c What's New In TimeTracker Basic? System Requirements For TimeTracker Basic: Mac OS X 10.5.8 or later (v10.5.8 and 10.6 are not supported). 64-bit PowerPC or Intel (32-bit PowerPC is not supported). 750 MHz or higher PowerPC or Intel processor. 2GB RAM or more. 750 MB or more free hard disk space. Internet access. Java 1.5 or later. Java Plug-in 1.5.2.3 or later. Also see the
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